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plant plasma membrane protein isolation kit  (Invent Biotechnologies)
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Invent Biotechnologies plant plasma membrane protein isolation kit
Western blotting IRT1 detection. (a) IRT1 <t>protein</t> abundance in aboveground parts of <t>plants</t> grown on perlite and watered with iron-containing (13.5 mg/L) or iron-depleted 1/10 MS solution. (b) Abundance of IRT1 protein in leaf rosette leaves (l) and roots (r) of seedlings grown in vitro on 1/2 MS or the same medium but with omitting iron sulfate. (c) IRT1 in different fractions of leaves and roots of seedlings grown in vitro on 1/2 MS or the same medium but with omitting iron sulfate. CYT = cytosol fraction, OM = organelle <t>membrane</t> fraction, PM = <t>plasma</t> membrane fraction
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Western blotting IRT1 detection. (a) IRT1 <t>protein</t> abundance in aboveground parts of <t>plants</t> grown on perlite and watered with iron-containing (13.5 mg/L) or iron-depleted 1/10 MS solution. (b) Abundance of IRT1 protein in leaf rosette leaves (l) and roots (r) of seedlings grown in vitro on 1/2 MS or the same medium but with omitting iron sulfate. (c) IRT1 in different fractions of leaves and roots of seedlings grown in vitro on 1/2 MS or the same medium but with omitting iron sulfate. CYT = cytosol fraction, OM = organelle <t>membrane</t> fraction, PM = <t>plasma</t> membrane fraction
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dna shuffling technique  (Nature Biotechnology)
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Nature Biotechnology dna shuffling technique
Western blotting IRT1 detection. (a) IRT1 <t>protein</t> abundance in aboveground parts of <t>plants</t> grown on perlite and watered with iron-containing (13.5 mg/L) or iron-depleted 1/10 MS solution. (b) Abundance of IRT1 protein in leaf rosette leaves (l) and roots (r) of seedlings grown in vitro on 1/2 MS or the same medium but with omitting iron sulfate. (c) IRT1 in different fractions of leaves and roots of seedlings grown in vitro on 1/2 MS or the same medium but with omitting iron sulfate. CYT = cytosol fraction, OM = organelle <t>membrane</t> fraction, PM = <t>plasma</t> membrane fraction
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la pcr in vitro mutagenesis kit  (TaKaRa)
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TaKaRa la pcr in vitro mutagenesis kit
Western blotting IRT1 detection. (a) IRT1 <t>protein</t> abundance in aboveground parts of <t>plants</t> grown on perlite and watered with iron-containing (13.5 mg/L) or iron-depleted 1/10 MS solution. (b) Abundance of IRT1 protein in leaf rosette leaves (l) and roots (r) of seedlings grown in vitro on 1/2 MS or the same medium but with omitting iron sulfate. (c) IRT1 in different fractions of leaves and roots of seedlings grown in vitro on 1/2 MS or the same medium but with omitting iron sulfate. CYT = cytosol fraction, OM = organelle <t>membrane</t> fraction, PM = <t>plasma</t> membrane fraction
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ampliscribetm t7-flashtm transcription kit  (Epicentre Biotechnologies)
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Epicentre Biotechnologies ampliscribetm t7-flashtm transcription kit
Western blotting IRT1 detection. (a) IRT1 <t>protein</t> abundance in aboveground parts of <t>plants</t> grown on perlite and watered with iron-containing (13.5 mg/L) or iron-depleted 1/10 MS solution. (b) Abundance of IRT1 protein in leaf rosette leaves (l) and roots (r) of seedlings grown in vitro on 1/2 MS or the same medium but with omitting iron sulfate. (c) IRT1 in different fractions of leaves and roots of seedlings grown in vitro on 1/2 MS or the same medium but with omitting iron sulfate. CYT = cytosol fraction, OM = organelle <t>membrane</t> fraction, PM = <t>plasma</t> membrane fraction
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rsk1 kinase activity in vitro  (Carna Inc)
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Carna Inc rsk1 kinase activity in vitro
Western blotting IRT1 detection. (a) IRT1 <t>protein</t> abundance in aboveground parts of <t>plants</t> grown on perlite and watered with iron-containing (13.5 mg/L) or iron-depleted 1/10 MS solution. (b) Abundance of IRT1 protein in leaf rosette leaves (l) and roots (r) of seedlings grown in vitro on 1/2 MS or the same medium but with omitting iron sulfate. (c) IRT1 in different fractions of leaves and roots of seedlings grown in vitro on 1/2 MS or the same medium but with omitting iron sulfate. CYT = cytosol fraction, OM = organelle <t>membrane</t> fraction, PM = <t>plasma</t> membrane fraction
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Image Search Results


Western blotting IRT1 detection. (a) IRT1 protein abundance in aboveground parts of plants grown on perlite and watered with iron-containing (13.5 mg/L) or iron-depleted 1/10 MS solution. (b) Abundance of IRT1 protein in leaf rosette leaves (l) and roots (r) of seedlings grown in vitro on 1/2 MS or the same medium but with omitting iron sulfate. (c) IRT1 in different fractions of leaves and roots of seedlings grown in vitro on 1/2 MS or the same medium but with omitting iron sulfate. CYT = cytosol fraction, OM = organelle membrane fraction, PM = plasma membrane fraction

Journal: Plant Signaling & Behavior

Article Title: Specific expression of AtIRT1 in phloem companion cells suggests its role in iron translocation in aboveground plant organs

doi: 10.1080/15592324.2021.1925020

Figure Lengend Snippet: Western blotting IRT1 detection. (a) IRT1 protein abundance in aboveground parts of plants grown on perlite and watered with iron-containing (13.5 mg/L) or iron-depleted 1/10 MS solution. (b) Abundance of IRT1 protein in leaf rosette leaves (l) and roots (r) of seedlings grown in vitro on 1/2 MS or the same medium but with omitting iron sulfate. (c) IRT1 in different fractions of leaves and roots of seedlings grown in vitro on 1/2 MS or the same medium but with omitting iron sulfate. CYT = cytosol fraction, OM = organelle membrane fraction, PM = plasma membrane fraction

Article Snippet: For membrane protein extraction, we used Minute™ Plant Plasma Membrane Protein Isolation Kit (Invent, Biotechnologies INC) following the manufacturer’s instructions.

Techniques: Western Blot, In Vitro